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Total RNA from biological replicates was extracted using the RNeasy Mini Kit (74104, Qiagen) according to the manufacturers instructions and analyzed by whole transcriptomic sequencing (Psomagen Inc), which returned RSEM counts and effective gene sizes

[191] English actor Dirk Bogarde once called Garland "the funniest woman I have ever met." [188] Despite her personal struggles, Garland disagreed with the public's opinion that she was a tragic figure

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If you salmon fillet is too big for container (for brining), slice into smaller serving portions